abstract：:The precipitation of a monoclonal IgG2 crystalline cryoglobulin (WEB) is shown to be highly dependent on temperature and concentration. Below a critical concentration of 0.6 mg/mL there is no cryoprecipitation. The kinetics of the aggregation exhibits a concentration-dependent lag time. This evidence suggests that a n...

journal_title：Biochemistry

authors： Scoville CD,Abraham GN,Turner DH

更新日期：1979-06-12 00:00:00

abstract：:Comparisons of membrane glycopeptides from baby hamster kidney fibroblasts (BHK21/C13) and a clone transformed by Rous sarcoma virus (C13/B4) were made by using cells metabolically labeled with radioactive D-glucose and L-fucose. Most of the glycopeptides were metabolically labeled with both the general and the specif...

journal_title：Biochemistry

authors： Glick MC

更新日期：1979-06-12 00:00:00

abstract：:Membrane vesicles from Mycobacterium phlei contain carrier proteins for proline, glutamine, and glutamic acid. The transport of proline is Na+ dependent and required substrate oxidation. A proline carrier protein was solubilized from the membrane vesicles by treatment with cholate and Triton X-100. Electron microscopi...

journal_title：Biochemistry

authors： Lee SH,Cohen NS,Jacobs AJ,Brodie AF

更新日期：1979-05-29 00:00:00

abstract：:The far-ultraviolet circular dichroism spectra of bovine and human prothrombin, prothrombin fragment 1, prethrombin 1, prothrombin fragment 2, and prethrombin 2 (prethrombin 2des(1--13)) were determined and the method of Chen et al. [Chen, Y. H., Yang, J. T., & Martinez, H. M. (1972) Biochemistry 11, 4120--4131; Chen,...

journal_title：Biochemistry

authors： Bloom JW,Mann KG

更新日期：1979-05-15 00:00:00

abstract：:The thermotropic behavior of aqueous dispersions of palmitoylsphingomyelin-cholesterol and lignoceryl-sphingomyelin-cholesterol mixtures has been examined by high-sensitivity differential scanning calorimetry. When less than 25 mol % cholesterol is mixed with either sphingomyelin, the calorimetric endotherm is compose...

journal_title：Biochemistry

authors： Estep TN,Mountcastle DB,Barenholz Y,Biltonen RL,Thompson TE

更新日期：1979-05-15 00:00:00

abstract：:Porcine aortic myosin is a smooth muscle contractile protein similar to its striated muscle counterpart. Electrophoresis in sodium dodecyl sulfate indicates that the molecule consists of three classes of subunits with polypeptide chain molecular weights of 192,000, 19,000, and 15,000. At 277 nm the absorption spectrum...

journal_title：Biochemistry

authors： Frederiksen DW

更新日期：1979-05-01 00:00:00

abstract：:The partition of cis-parinaric acid (9,11,13,15-cis, trans, trans,cis-octadecatetraenoic acid, cis-PnA) and trans-parinaric acid (9,11,13,15-all-trans-octadecatetraenoic acid, trans-PnA) among aqueous, solid lipid, and fluid lipid phases has been measured by three spectroscopic parameters: absorption spectral shifts, ...

journal_title：Biochemistry

authors： Sklar LA,Miljanich GP,Dratz EA

更新日期：1979-05-01 00:00:00

abstract：:Membrane vesicles isolated from Escherichia coli ML 308--225 have been analyzed by crossed immunoelectrophoresis, and immunoprecipitates corresponding to the following cellular components have been identified: ATPase (EC 3.6.1,3), two or three NADH dehydrogenases (EC 1.6.99.3), D-lactate dehydrogenase (EC 1.1.1.27), g...

journal_title：Biochemistry

authors： Owen P,Kaback HR

更新日期：1979-04-17 00:00:00

abstract：:The plasma membrane of the Ehrlich ascites tumor cell contains an NADH dehydrogenase. This activity was shown not to be due to contamination by other subcellular membranes. A variety of electron acceptors have been compared as to rate with the following result: ferricyanide greater than cytochrome c greater than cytoc...

journal_title：Biochemistry

authors： Kilberg MS,Christensen HN

更新日期：1979-04-17 00:00:00

abstract：:The structure of premature core enzyme, an obligatory intermediate in both in vivo and in vitro assembly of Escherichia coli DNA-dependent RNA polymerase, was compared with that of native core enzyme. Though this assembled but inactive form of core enzyme harbors the gross conformation similar to that of native enzyme...

journal_title：Biochemistry

authors： Ishihama A,Aiba H,Saitoh T,Takahashi S

更新日期：1979-03-20 00:00:00

abstract：:The micellar properties of mixed, bovine gangliosides and purified galactosyl-N-acetylacetylgalactosaminyl (N-acetylneuraminyl) galactosylglucosylceramide were studied by gel filtration, equilibrium dialysis, and band and boundary centrifugation in sucrose gradients. The dissociation of micelles is very slow (days) in...

journal_title：Biochemistry

authors： Formisano S,Johnson ML,Lee G,Aloj SM,Edelhoch H

更新日期：1979-03-20 00:00:00

abstract：:To probe the details of small amplitude motions in proteins, a dynamical analysis of the orientation fluctuations of two tyrosine side chains in the bovine pancreatic trypsin inhibitor is presented. Detailed results are given for the time history and correlation functions obtained for the ring motion from a molecular ...

journal_title：Biochemistry

authors： McCammon JA,Wolynes PG,Karplus M

更新日期：1979-03-20 00:00:00

abstract：:The addition of 10(-9) M insulin to a suspension of rat liver plasma membranes increases the overall lipid microviscosity, eta, by about 10--20%. The effect is confined to physiological concentrations of the hormone and is highly specific. The specificity was demonstrated in experiments where insulin analogues were ad...

journal_title：Biochemistry

authors： Luly P,Shinitzky M

更新日期：1979-02-06 00:00:00

abstract：:At pH 5.5, binding of bleomycin relaxed supercoiled ColE1 DNA without breaking it. Binding of tripeptide S, a fragment of the drug containing the bithiazole rings, also relaxed and then recoiled supercoiled DNA, at pH 5.5 and at pH 8.0, where bleomycin is normally active. The unwinding angle was 12 degrees. Both compo...

journal_title：Biochemistry

authors： Povirk LF,Hogan M,Dattagupta N

更新日期：1979-01-09 00:00:00

abstract：:Studies of the mechanism of protein secretion in a Gram-positive bacterium, Bacillus subtilis, yielded results very similar to those previously obtained with a Gram-negative organism: nascent chains protruding from protoplasts could be labeled extracellularly; the labeled chains could be recovered on polysomes isolate...

journal_title：Biochemistry

authors： Smith WP,Tai PC,Davis BD

更新日期：1979-01-09 00:00:00

abstract：:Purified beta subunit of human chorionic gonadotropin (hCG) was partially reduced with beta-mercaptoethanol, carboxymethylated, and digested with chymotrypsin. The peptides were isolated by high-voltage electrophoresis and paper chromatography. Five major disulfide-containing peptides were isolated, and their location...

journal_title：Biochemistry

authors： Swaminathan N,Braunstein GD

更新日期：1978-12-26 00:00:00

abstract：:The amino acid sequence of the peptide alpha1(I)-CB6B, the carboxyl-terminal peptide containing 91 residues of alpha1(I) chain of chick skin collagen, is described. The amino acid sequence was determined by automated Edman degradation of intact peptide plus its tryptic and thermolytic peptides. The comparison of this ...

journal_title：Biochemistry

authors： Dixit SN,Seyer JM,Kang AH,Gross J

更新日期：1978-12-26 00:00:00

abstract：:The electric organ of the ray, Torpedo ocellata, can serve as a source for both the acetylcholine (ACh) receptor and its ionic channel. The two entities were identified by their specific binding of [3H]ACh and [3H]perhydrohistrionicotoxin ([3H]H12-HTX), respectively. Binding of [3H]H12-HTX was inhibited by certain dru...

journal_title：Biochemistry

authors： Eldefrawi ME,Eldefrawi AT,Mansour NA,Daly JW,Witkop B,Albuquerque EX

更新日期：1978-12-12 00:00:00

abstract：:The effect of ionic strength, temperature, and divalent cations on the association of myosin with actin was determined in the ultracentrifuge using scanning absorption optics. The association constant (Ka) for the binding of heavy meromyosin (HmM) to F-actin was 1 X 10(7) M-1 at 20 degrees C, in 0.10 M KCl, 0.01 M imi...

journal_title：Biochemistry

authors： Margossian SS,Lowey S

更新日期：1978-12-12 00:00:00

abstract：:The selective reaction of SH containing proteins and peptides with NTCB (2-nitro-5-thiocyanobenzoic acid) has been reported (Degani, Y., & Patchornick, A. (1974) Biochemistry 13, 1; Jacobson, G.A., Schaffer, M.H., Stark, G.R., & Vanaman, T.C. (1973) J. Biol. Chem. 248, 6583). With this reagent, cysteinyl peptide bonds...

journal_title：Biochemistry

authors： Otieno S

更新日期：1978-12-12 00:00:00

abstract：:T4 RNA ligase catalyzes the ATP-dependent addition of a single 2'-deoxyribonucleoside 3',5'-bisphosphate to the 3'-hydroxyl of an oligodeoxyribonucleotide. The bisphosphate is joined to the deoxyoligomer by a 3' leads to 5' phosphodiester bond and the product, which is terminated by a 3'-phosphate, is one nucleotide l...

journal_title：Biochemistry

authors： Hinton DM,Baez JA,Gumport RI

更新日期：1978-11-28 00:00:00

abstract：:The bile acid cholate disrupts platelet plasma membrane vesicles and inactivates 5-hydroxytryptamine transport activity. The transporter appears not to be solubilized by this procedure, but remains associated with a high molecular weight aggregate. Merely removing cholate does not restore the vesicular structure or tr...

journal_title：Biochemistry

authors： Rudnick G,Nelson PJ

更新日期：1978-11-28 00:00:00

abstract：:Carboxymethylation with 14 C-labeled iodoacetate of cysteine residues in wild-type beta-galactosidase from Escherichia coli and in a defective beta-galactosidase from deletion mutant strain M15 was investigated in order to determine accessible positions in the tetrameric wild-type form and the dimeric mutant M15 prote...

journal_title：Biochemistry

authors： Jörnvall H,Fowler AV,Zabin I

更新日期：1978-11-28 00:00:00

abstract：:Diffusion studies by photon correlation of scattered laser light confirm the dissociation of the tetrameric form of human carboxyhemoglobin to dimers above pH 10 and provide new estimates of the subunit dissociation equilibrium constants in this pH range. Electrophoretic light-scattering experiments under the same con...

journal_title：Biochemistry

authors： Haas DD,Ware BR

更新日期：1978-11-14 00:00:00

abstract：:The binding of ethidium bromide to the self-complementary deoxytetranucleotides containing guanine and cytosine bases has been studied by circular dichroism, visible absorption, and fluorescence spectroscopies. The circular dichroism spectrum of each of the four deoxytetranucleotides was measured and compared to the s...

journal_title：Biochemistry

authors： Kastrup RV,Young MA,Krugh TR

更新日期：1978-11-14 00:00:00

abstract：:The binding of Lens culinaris lectin to the receptors of rabbit erythrocytes was studied by a quantitative fluorometric method using the fluoresceinated conjugate. Equilibrium data showed the presence of 1.2 x 10(6) receptors/cell with an association constant varying from 8 to 3 x 10(6) M-1 over a temperature range of...

journal_title：Biochemistry

authors： Hoebeke J,Foriers A,Schreiber AB,Strosberg AD

更新日期：1978-11-14 00:00:00

abstract：:alpha-Glucosidase (EC 3.2.1.20) was purified to homogeneity from logarithmically growing cells of Saccharomyces carlsbergensis. The purification involved the following steps: (a) ammonium sulfate fractionation; (b) Sephadex G-100 chromatography; (c) DEAE-cellulose chromatography; and (d) hydroxylapatite chromatography...

journal_title：Biochemistry

authors： Needleman RB,Federoff HJ,Eccleshall TR,Buchferer B,Marmur J

更新日期：1978-10-31 00:00:00

abstract：:Resonance Raman spectra of squid rhodopsin have been obtained under a variety of temperature and illumination conditions. The data have been characterized in terms of spectral contributions from squid rhodopsin, isorhodopsin, bathorhodopsin, lumirhodopsin, mesorhodopsin, P-465, and acid metarhodopsin. The results are ...

journal_title：Biochemistry

authors： Sulkes M,Lewis A,Marcus MA

更新日期：1978-10-31 00:00:00

abstract：:Chemical-modification studies on submitochondrial particle pyridine dinucleotide transhydrogenase (EC 1.6.1.1) demonstrate the presence of one class of sulfhydryl group in the nicotinamide adenine dinucleotide phosphate (NADP) site and another peripheral to the active site. Reaction of the peripheral sulfhydryl group ...

journal_title：Biochemistry

authors： Earle SR,O'Neal SG,Fisher RR

更新日期：1978-10-31 00:00:00

abstract：:The existence of two conformational states of concanavalin A (Con A) with different metal ion binding properties has been recently demonstrated (Brown, R. D., Brewer, C. F., & Koenig, S. H. (1977) Biochemistry 16, 3883). Introduction of Mn2+ to the S1 site and Ca2+ to the S2 site of apo-Con A was shown to induce a con...

journal_title：Biochemistry

authors： Koenig SH,Brewer CF,Brown RD 3rd

更新日期：1978-10-03 00:00:00

abstract：:The complete sequence of rabbit antibody light-chain L 120 has been elucidated. The antibody was raised against Micrococcus lysodeikticus bacteria and is specific for the external part of the cell wall. All protein used in this work was obtained from a single 50-mL bleeding. The variable region of L 120 is compared to...

journal_title：Biochemistry

authors： Van Hoegaerden M,Strosberg AD

更新日期：1978-10-03 00:00:00

abstract：:The behavior of Ca2+-ATPase from sarcoplasmic reticulum in detergent solution was compared with that of Ca2+-ATPase which had been cleaved in half by limited trypsin digestion. Attempts to dissociate the fragments (I and II) with an excess of detergent micelles demonstrated that fragments I and II are structurally dep...

journal_title：Biochemistry

authors： Rizzolo LJ,Tanford C

更新日期：1978-09-19 00:00:00

abstract：:Both activities of the aspartokinase--homoserine I (AK-HSD) of Escherichia coli are inhibited by threonine. Careful threonine binding studies have now been done which have allowed us to distinguish the various effects of threonine on the enzyme. The ultrafiltration technique of H. Paulus ((1969) Anal. Biochem. 32, 101...

journal_title：Biochemistry

authors： Bearer CF,Neet KE

更新日期：1978-08-22 00:00:00

abstract：:The specificity of valyl-, phenylalanyl-, and tyrosyl-tRNA synthetases from yeast has been examined by a series of stringent tests designed to eliminate the possibility of artefactual interference. Valyl-tRNA synthetase, as well as activating a number of amino acid analogues, will accept alanine, cysteine, isoleucine,...

journal_title：Biochemistry

authors： Igloi GL,von der Haar F,Cramer F

更新日期：1978-08-22 00:00:00

abstract：:The transformation of DMPC vesicles containing myristic acid to larger structures was examined by nuclear magnetic resonance techniques. The process was found to be characterized by a rate which is approximately first order in vesicle concentration. Rates, however, show a dramatic increase with increasing myristic aci...

journal_title：Biochemistry

authors： Kantor HL,Prestegard JH

更新日期：1978-08-22 00:00:00

abstract：:Five previously unknown hemorrhagic proteins, designated hemorrhagic toxins a,b,c,d, and e, were isolated from the venom of the western diamondback rattlesnake (Crotalus atrox). Molecular weights of hemorrhagic toxins a-e were determined to be 68 000, 24 000, 24 000, 24 000, and 25 700, respectively, by sodium dodecyl...

journal_title：Biochemistry

authors： Bjarnason JB,Tu AT

更新日期：1978-08-08 00:00:00

abstract：:The pH dependence of buffer catalysis of exchange of the C-4 amino protons of cyclic cytosine 2',3'-monophosphate (cCMP) and the N-1 proton of cyclic guanosine 2',3'-monophosphate (cGMP) conforms to an exchange mechanism, in which protonation of the nucleobases at C(N-3) AND G(N-7) establishes the important intermedia...

journal_title：Biochemistry

authors： McConnell B

更新日期：1978-07-25 00:00:00

abstract：:Alpha2u-globulin is a male rat liver protein under multihormonal control which represents approximately 1% of hepatic protein synthesis. We have measured the rate of transcription of the alpha2u-globulin gene using a nuclear cell-free transcriptional system with mercurated CTP as substrate for the endogenous RNA polym...

journal_title：Biochemistry

authors： Chan KM,Kurtz DT,Feigelson P

更新日期：1978-07-25 00:00:00

abstract：:A rapid assay procedure was developed for cleavage of the N-terminal propeptides of procollagen. With the assay a neutral procollagen N-protease was purified about 300-fold from chick embryo tendon extract. The enzyme had an apparent molecular weight of 260 000 and a pH optimum of 7.4. Ca2+ was required for enzymic ac...

journal_title：Biochemistry

authors： Tuderman L,Kivirikko KI,Prockop DJ

更新日期：1978-07-25 00:00:00

abstract：:The level of albumin mRNA in the normal Buffalo rat liver and Morris hepatoma 7777 was compared by molecular hybridization with albumin complementary DNA (cDNA) and translational assays. Albumin mRNA was found to be 10% of the total rat liver poly(A)-containing RNA population but reduced approximately fourfold in the ...

journal_title：Biochemistry

authors： Tse TP,Morris HP,Taylor JM

更新日期：1978-07-25 00:00:00